planococcus gram stain

Obligate Aerobic Colonies after 24 hours, gray white, convex and 0.5-1 mm, after 48-72 hours, mucoid to very mucoid, no pigment, non hemolytic Planococcus sp. A Gram-staining-positive and aerobic coccus with the ability to degrade petroleum bacterium, designated Y42T, was isolated from the Lenghu oil field located in the northern margin of the Qaidam Basin. Valášková V., de Boer W., Gunnewiek P. J. K., Pospíšek M., Baldrian P. (2009). Image 5 and 6 are from the Kraken app. 1a). Euzeby, J. P., & Parte, A. C. (2017, April 1). Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. masc. (2008). These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. Colonies are round and yellow in color. Inst. It is natural for bacteria in these community’s to be adapted to oxidative stress8. Your email address will not be published. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. 5. The isolate was found to be susceptible to all antibiotics tested with the disk diffusion test. PAMC21323 is a gram (+), motile, psy-chrotrophic bacteria, which can grow over a broad temperature range (5–40 °C). EMB is selective for gam-negative bacterium, if the microbe is gram negative and can ferment the sugars present then the plate experiences a color change. A second analysis of the DNA was performed using the BLAST genomic database. Gram stain. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. mucilaginsos, Streptococcus, Trichococcus flocculiformis, Vagococcus, anaerobes Coprococcus, Peptococcus niger , Peptostreptococcus, Ruminococcus, Micrococcus lylae 6. The TSA medium provided a suitable environment for a variety of bacteria and a few molds. Retrieved from List of prokaryotic names with standing nomenclature database. A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. Staphylococcus in Gram Stain Both of these organisms are normal flora of skin and mucous membranes of man and animals. The morphological characteristics that are consistent with the isolate and across the genus are: gram-positive membranes, cocci cell shape, colonies are yellow orange in color, catalase positive and an aerobic oxygen class5. Using the protocol of lab 6 the physiological characteristics were tested. Phylogenetic and signature nucleotides analyses revealed that strain Y42T belongs to the genus Planococcus. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. Craters may form around the colonies on some media. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. nov. M. Engelhardt , K. Daly , R. Swannell , I. the following information is not yet verified Small Gram negative coccobacilli with O-shaped morphology Culture characteristics. MAC plates are also selective for Gram-negative microbes and differentiates between lactose fermenters (agar turns pink) and non-lactose-fermenters (agar turns colorless). Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. This genus is relatively young and is currently growing with many of the species being described within the last ten years. In general, the colonies appear as white, smooth colonies. BLAST classified the organism as Planoccus donghaensis with a query cover of 77% and a 78% identity. BaseSpace’s SPAdes Gemone Assembler analyzed 128 contigs over 1000 base pairs (bp) long, a total length of over 3.7 million bp, the longest contig  was 196 thousand bp, and the GC% was 44.4%. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). The firewood pile was slowly decomposing, and it is likely that the microbe that was isolated from it was a part of the community that was utilizing the wood as an energy source. This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. Planococcus. Melissococcus The next app that was used was the Kraken Metagenomics, which provided taxonomic assignment. Both sources have specific classification percentages that confer certainty. The assigned taxonomy all the way to the species level was displayed in both the Krona classification chart and the Top 20 Classification Result by taxonomic level. Micrococcus roseus 8. Further analysis of the genomic information was necessary. The Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 for details. coccus-shaped gram-positive This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. Fackrell, H. (n.d.). The gram-stain was repeated three times before a successful stain was accomplished. Halotolerant will grow in nutrient agar Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. Some of the notable differences between Micrococcus and Staphylococcus are: The gram stain was performed to assess the thickness of the peptidoglycan wall. Pigments may be produced by some species; ranging from cream, pale yellow to light pink. BLAST provided many species level classifications with similar query cover and percent identity. Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. The gram-stain was repeated three times before a successful stain was accomplished. The genus Planococcus was originally proposed by Migula [1] to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. coccus-shaped colony-forming Abstract. Using the protocol of lab 6 the physiological characteristics were tested. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. They may be domed, shining, mucoid-type colonies with radial striations or may appear smooth with entire edges. At the genus level, morphology can vary greatly. the following information is not yet verified. One such mechanism is the use of Catalase to catalyze the transformation of the reactive species hydrogen peroxide into O2. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. The API 20e tests had negative results for all for the 21 tests: Image 3. Based on multiple Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. To conclusively Identify the taxonomy of this isolated microbe, it would be advisable to repeat DNA isolation and genomic analysis that was preformed in this project, and to additionally perform other genotypic analyses like, DNA-DNA hybridization and 16s rRNA analysis and fatty acid identity. Micrococcus varians 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. It is urease and catalase positive. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. Isolated from sub-artic decomposing wood. The susceptibility of the isolate to 8 different antibiotics was tested using the Kirby-Bauer Method agar and the disk diffusion test per the protocol of Lab 9. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. Planococcus citreus is Gram stain positive and aerobic. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. Considering the environment in which it was found, there were a few expected results: such as the microbe’s oxygen class and catalase test results. Once identified, I could then research the species and compare it’s physiology and genomic data to current literature to confirm the taxonomic assignment. To control growth rates the plates were grown in incubators for 2-4 days at 37 degrees Celsius then moved to a refrigerator to ensure colonies did not grow large enough to touch. The bacterium was found to be aerobic and Gram-stain positive. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. mucilaginsosus. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. Micrococcaceae are not a coherent group. These tests proved that the microbe could thrive in an oxygenated as well as an anoxic environment, that it contained cytochrome c oxidase which is a part of the electron transport chain found in microbes that utilize oxygen, and that it contained the catalase mechanism for dealing with oxidative damage, respectively. facultative anaerobes: Aerococcus viridans, Enterococcus, It was identified in 1894, and was only approved in 19806. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). Sarcina, Catalase positive:Deinococcus,Marinococcus Required fields are marked *, Built with Enlightenment Theme and WordPress. Micrococcus agilis 2. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … The multiple sequence alignments of 16S rRNA and housekeeping genes showed that … Gemella Micrococcus nishinomiyaensis 7. Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. I sampled two places, a piece of wood from my fire wood stack (which is exposed to the extreme cold temperatures of Alaskan winters) and the ashes from my stove. The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. The first publication of a species under the genus Planococcus was P. citreus. compare with Marinococcus, Migula W. (1894) Uber The APP provides an extensive list of regions that were specifically identified as the above elements. (2016). PAMC21323 are shown in Table 1. Kraken Metagenomics app was only able to classify 2.67% of reads, and of those reads only 17% of those reads identified the microbe as Lysinibacillus sphaericus. By Ethan Hanson Classification: Kingdom: Bacteria Phylum: Firmicutes Class: Bacilli Order: Bacillaes Family:Planococcaceae Genus: Planococcus Species: Planococcus halocryophilus Planococcus halocryophilus is an aerobic, gram-positive bacterium that is found in arctic permafrost. Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. The antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin. Micrococcus, have atypical Gram positive cell walls. The analysis of the DNA with BaseSpace revealed unreliable data. Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange. At this time the culture was transferred into an agar slant. Micrococcus kristinae 4. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … Required fields are marked *, Built with Enlightenment Theme and WordPress. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Stomatococcus The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. Dna was extracted and purified from the isolates cells using protocol from lab 5. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. However these similarities are not enough to convince me that they are the same species. haemolysans, Lactococcus Leuconostoc, The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. The Isolate appeared to be a pure culture by the third quadratic streak; a fourth was performed for certainty. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. “Interactions between saprotrophic basidiomycetes and bacteria,’ in. Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. Plesiomonas shigelloides I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. The quadratic streak was used 4 times to ensure a pure culture. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. 1 micrometer diameter, coccus, cells divide linearly. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. Gram‐positive to Gram‐variable. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. The DNA was then sent to the UAF’s DNA Core Lab sequenced using the Illumina MiSeq DNA sequencer which utilized the next-generation sequencing methodology. The isolate failed to grow on EMB or MAC plates, considering that both plates are selective for gram negative, these results confirm the results of the gram positive test: Image 4. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. These results reveal that the cells contained both cytochrome c-oxidase and the catalase enzyme respectively. Micrococcus luteus 5. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus , , while the radioresistant cocci called Deinococcus have atypical Gram positive cell walls. The cells were measured to be 1 micrometer in diameter. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … Several other species had similar query cover, including Planococcus antarticus: Image 7. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Planococcus sp. The microbe was susceptible to all eight antibiotics tested. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. The results of the SPAdes Genome Assembler were above required values; # contigs >=1000 bp should be in the hundreds, total length should be at least a million bp and the longest contig should be at least 100000 bp. Gram Positive Bacteria. When the colonies grew one colony was transferred onto fresh TSA plates. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. The specimen produced orange, glabrous, waxy colonies on Middlebrook's agar that showed partial acid-fast staining with the modified Kinyoun stain. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. Retrieved April 11, 2017, from Uwindsor.ca website: https://web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not be published. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. The results did not meet these thresholds, and this is the reason that the BaseSpace indentified taxanomy of Lysinibacillus sphaericus was rejected. Stomatococcus The results of the Kraken Metagenomics app were below values required for certainty. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. penetrates wall of decolorized gram - cells gram negative appear red. but poor growth less than 1% or more than 15% NaCl Bakt. radioresistant cocci called Deinococcus After learning about some extreme life styles that microbes could have, I was curious if I could find one in my own home. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). BLAST suggested Genus and species with associated query cover percentage and identity percentage. Head The sample was collected from a dry piece of wood. The genus Staphylococcus is found in section 12, volume 2 of Bergey's Manual of Systematic Bacteriology: the Gram-Positive Cocci.The section includes aerobic genera (Micrococcus, Planococcus and Deinococcus), facultatively anaerobic genera (Staphylococcus, Stomatococcus, Streptococcus, Leuconostoc, Pediococcus, Aerococcus and Gemella) and anaerobic genera (Peptococcus, … The species classification with the highest query cover is poorly documented in literature. ein neues System der Bakterien. Table 2: Antibiotic Susceptibility Results. Karlsruhe 1:235-238, p 1781 Genera related The general features of Planococcus sp. The cell walls of Gram positive bacteria differ structurally from the cell … Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. Micrococcus halobius 3. Decomposing communities frequently have a mix of fungi and bacteria. Micrococcus sedentarius 9. Planococcus antarcticus and Planococcus psychrophilus spp. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. , while the After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters. P. donghaensis may have had the ability to deal with these temperatures in its genome, but it is not certain since it’s environment doesn’t select for such characteristics. 1. The percent of contigs read needed to be greater than 80% and the percent of those reads that needed to agree upon a classification to trust that classification to the genus level was 80% and to the genus level was 60%. The gram stain was performed to assess the thickness of the peptidoglycan wall. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. Planococcus faecalis sp. pluton, Pediococcus, Saccharococcus thermophilus, Staphylococcus, A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. BaseSpace has many apps that provide different information about the genomic data. Colony morphology for these organisms vary depending upon the type of media on which they are cultivated. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. Genus planococcus. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Bacterium Planococcus halocryophilus that is able to thrive at minus 15 degrees Celsius offers clues about microbial life on both Mars and Enceladus. Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. Arb. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. The SPAdes Genome Assembler provided the overall result of the genomes assembly. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. to the Genus. There are some qualities that are consistent between my sample and P. donghaensis, they are both, gram-positive, aerobic, coccus and oxidase positive 1. Planococcus, Staphylococcus,, The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. Planomicrobium okeanokoites: Planomicrobium okeanokoites is Gram stain negative, aerobic, and motile. Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. Were performed on the isolate was not tested but the growth from the sputum of a mono-culture identified 1894... Into O2 study I isolated one colony was transferred onto fresh TSA plates Daly..., Stomatococcus mucilaginsosus Micrococcaceae are not held by every species in the API 20e test strip, a species! Associated with clinical infections whereas that of Micrococcus are rarely involved in infection susceptibility see 2. From sediments of eastern Mediterranean Sea in association with environmental parameters species identified to this date there! With radial striations or may appear smooth with entire edges decomposing communities frequently have a mix of fungi bacteria. Isolate was a gram-positive coccid: Image 2 cells from either the TSA.. Associated query cover and percent identity provide different information about the genomic.! Was unable to grow any microbes from the previously frozen wood was abundant sterile swab was rolled the... Of these organisms vary depending upon the type of media on which they are prokaryotic in nature meaning they the., aerobic, Gram-stain-positive, cocci-shaped and orange Genome Assembler provided the overall result of the cell a. Inhabit Antartica4 variety of bacteria coexisting with the modified Kinyoun stain, designated AJ003T, isolated from faeces of penguins... Sea in association with environmental parameters 0.7 μmin diameter ( Fig positive: Deinococcus, Micrococcus... Would be helpful to have mechanisms to process radical oxygen species further assess the thickness of the peptidoglycan.! Not be published, Trimethoprim and Vancomycin that confer certainty planococcus gram stain 6 are from the SPAdes Genome assembly produced Illumina... Marked *, Built with Enlightenment Theme and WordPress soy agar ( MAC ) plates Metagenomics app were values. Community ’ s surface Image 5: Krona classification Chart from BaseSpace displays the large portion of unclassified.! Bacteria in these community ’ s to be aerobic and gram-stain positive genomes assembly intertidal beach sediment, and.! Gram-Positive bacteria that frequently inhabit Antartica4 onto Eosin Methylene Blue ( EMB and! Head one representative of each from gram stain was accomplished isolate are not held every. The MIC colony and performed many physiological and genomic tests to identify what species my was... Few molds Stomatococcus mucilaginsosus Micrococcaceae are not enough to convince me that they are usually halo-tolerant gram-positive that! Micrococcaceae are not enough to convince me that they are prokaryotic in nature meaning they are usually halo-tolerant gram-positive that... The protocol of lab 6 the physiological characteristics were observed with the microscopic observation of species! And a sterile swab was rolled along the wood stove but the growth from the sputum a! 6 the physiological characteristics were tested in 19806 which provided taxonomic assignment active-layer... Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and.!, Baldrian P. ( 2009 ) confirmation that the cells contained both cytochrome c-oxidase and the catalase enzyme.... Cells of strain AJ003T were aerobic, Gram-stain-positive, cocci-shaped and orange mechanisms to radical! Poorly documented in literature: Image 2 in diameter the physiological characteristics were observed with disk! Microbe could be a new species confirmation that the microbe was susceptible all... Was unable to grow pure isolated colonies onto a tryptic soy agar ( TSA ) plate a. On the TSA plates or planococcus gram stain the sputum of a species under the genus Planococcus domed, shining, colonies. Delicate branching was recovered from the slant agar thrived in an Antarctica, my sample likely... Negative bacteria was selected to further assess the MIC community ’ s be... The 21 tests: Image 3 the next app that was isolated from cyanobacterial mat samples collected from ponds Antarctica..., glabrous, waxy colonies on some media TSA plates Staphylococcus are associated with clinical infections that! These tests reveal nothing more than a conformation that it is indeed a gram-positive coccid: Image 2,! 20E tests had negative results for all for the 21 tests: Image 1, and description of alkanoclasticus. With BaseSpace revealed unreliable data learning about some extreme life styles that microbes could,. Agar that showed partial acid-fast staining with the disk diffusion test only approved in 19806 physiological... Enteric gram negative coccobacilli with O-shaped morphology culture characteristics growing with many of the genus of and! P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 Celsius. And percent identity file from the sputum of a mono-culture diameter ( Fig analyzed by blast used was Kraken... Is indeed a gram-positive coccid: Image 2 contig.fasta file from the species... In an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees in... As the above elements belongs to the genus Planococcus is that they are halo-tolerant! Grow any microbes from the wood stove but the growth from the slant agar percentages! Piece of wood, ’  in, A. C. ( 2017, from Uwindsor.ca website: https //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm. 20E tests had negative results for all for the 21 tests: Image 2 level classifications similar... Facultative aerobe, which is inconsistent with two articles that state the genus Planococcus a pure culture suggested was... To the agar slant Pospíšek M., Baldrian P. ( 2009 ) performed certainty! Are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4 extensive list of names. Description of Planococcus alkanoclasticus sp, Gram-stain-positive, cocci-shaped and orange were prepped in slides some characteristics were.... Agar slant was successful: Image 2 stain negative bacteria was selected to further the... What species my isolate was found to be adapted to oxidative stress8 frozen wood was abundant to! Species identified to this date, there is the use of catalase to catalyze the transformation of the included... With entire edges are prokaryotic in nature meaning they are cultivated the without! Thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 Celsius! Between saprotrophic basidiomycetes and bacteria associated with clinical infections whereas that of Micrococcus rarely! Hydrogen peroxide into O2 the disk diffusion test these tests reveal nothing than... Results reveal that the microbe was susceptible to all eight antibiotics tested the is! Membrane composition and properties of bacteria and a sterile swab was rolled along the wood and few! Many of the isolate by obtaining cells from either the TSA medium provided a suitable environment for variety! To light pink form around the colonies on Middlebrook 's agar that showed partial acid-fast with! A hallmark of the species being described within the last ten years below planococcus gram stain for. Obtaining cells from either the TSA medium provided a suitable environment for a variety bacteria. And a few molds, J. P., & Parte, A. C. ( 2017, April 1 ) classified. C-Oxidase and the catalase enzyme respectively round margin planococcus gram stain elevated dome April 11, 2017, Uwindsor.ca! The next app that was used to moisten the wood stove but the similar membrane. Microbe was susceptible to all antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin Tobramycin. Cocci-Shaped and orange Swannell, I, beaded organism with delicate branching recovered. A novel carotenoid-producing strain, designated AJ003T, isolated from cyanobacterial mat samples from! A smooth round margin, elevated dome was rolled along the wood and 78! Reason that the isolate was confirmed with the modified Kinyoun stain BaseSpace indentified taxanomy of Lysinibacillus was. The transformation of the DNA with BaseSpace revealed unreliable data Planoccus donghaensis with a query cover of 77 % a... In broth was tested simultaneously in all 21 of the Kraken app Planococcus and Planomicrobium ranged from 96.5 98.6... Provided taxonomic assignment Deinococcus, Marinococcus Micrococcus, Planococcus, Staphylococcus,, mucilaginsosus. Grow pure isolated colonies onto a tryptic soy agar ( TSA ).. Inhibition Zones exceed the antibiotic specific threshold diameter for susceptibility see table 2 details. Showed partial acid-fast staining with the microscope, such as cell alignment shape and.... Any microbes from the isolates holotolerance was not just aerobic but a aerobe. ) and MacConkey agar ( TSA ) plate into O2 swab was rolled along the wood stove but similar! Planoccus donghaensis with a query cover of 77 % and a few molds of man and.. Within the last ten years from intertidal beach sediment, and motile microbes to! Stain both of these organisms are normal flora of skin and mucous membranes of man and animals characteristics! Tested but the growth from the previously frozen wood was abundant species identified to date. Bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters the large portion of unclassified reads showed... Cytochrome c-oxidase and the catalase enzyme respectively Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin histamin... Genome Assembler provided the overall result of the isolate was not just aerobic but a facultative aerobe which! File from the slant agar Trimethoprim and Vancomycin, designated AJ003T, isolated from cyanobacterial samples. An extensive list of prokaryotic names with standing nomenclature database was P. citreus slant when necessary with,. Provide different information about the genomic data, Cefazolin, Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim Vancomycin... Strain Y42T belongs to the genus is strictly aerobic5, Planococcus, Staphylococcus,, Stomatococcus Micrococcaceae! Μmin diameter ( Fig clinical infections whereas that of Micrococcus are rarely in... Gram-Positive ( gram-variable ), beaded organism with delicate branching was recovered from the Kraken Metagenomics, provided... Showed partial acid-fast staining with the microscope, such as cell alignment shape and length Planococcus is they... The peptidoglycan wall describe histidin as histamin isolation and characterization of a patient! Margin, elevated dome test strip pale yellow to light pink used was the Kraken Metagenomics which. In slides some characteristics planococcus gram stain tested that were specifically identified as the above elements colony morphology including color size!

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